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产品名称:GenJet (Ver. II) DNA In Vitro Transfection Reagent for NIH3T3 Cell
功能:
GenJet™ DNA In Vitro Tranfection Reagent for NIH3T3 Cells is pre-optimized for NIH3T3 cell transfection. NIH 3T3 cells come from a cell line established in 1962 by two scientists then at the Department of Pathology in the New York University School of Medicine, George Todaro and Howard Green. The 3T3 cell line has become the standard fibroblast cell line. Todaro and Green originally obtained their 3T3 cells from Swiss mouse embryo tissue.
最佳转染条件:
Confluency of cell on the day of shaving 70%
GenJet? (μl) : DNA (μg) Ratio 3:1
Diluent for DNA and Transfection Reagent Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet?/DNA Complex 15 minutes at RT
Maximal Efficiency 48 hours
Transfection Results:
Reporter Gene EGFP
Plasmid pEGFP-N3 (CMV promoter)
Efficiency (GFP %) 90%
储存条件:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer. |