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产品名称:GenJet (Ver. II) DNA In Vitro Transfection Reagent for HepG2 Cell
功能:
GenJet™ DNA In Vitro Tranfection Reagent for HepG2 Cells is pre-optimized for HepG2 cell transfection. HepG2 (Hepatocellular carcinoma, human) is a perpetual add cell line which was derived from the liver tissue of a 15 year old caucasian male with a well differentiated hepatocellular carcinoma. These cells are epithelial in morphology, have a model chromosome number of 55 and are not tumorigenic in nude mice. The cells secrete a variety of major plasma proteins e.g.. albumin, alpha 2-macroglobulin, alpha 1-antitrypsin, transferrin and plasminogen. They have been grown successfully in large scale cultivation systems. Hepatitis B virus surface antigens have not been detected. HepG2 cells have been shown to be G418 resistant (400µg/mL). The cells will respond to stimulation with human growth hormone.
最佳转染条件:
Confluency of cell on the day of shaving 90%
GenJet? (μl) : DNA (μg) Ratio 3:1
Diluent for DNA and Transfection Reagent Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet?/DNA Complex 15 minutes at RT
Maximal Efficiency 48 hours
Transfection Results:
Reporter Gene EGFP
Plasmid pEGFP-N3 (CMV promoter)
Efficiency (GFP %) 82%
储存条件:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer. |