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产品名称:GenJet (Ver. II) DNA In Vitro Transfection Reagent for COS Cell
功能:
GenJet™ DNA In Vitro Tranfection Reagent for COS Cells is pre-optimized for COS cell transfection. COS is a cell line often used by biologists when studying the monkey virus SV40. It is also often used to transfect cells to produce recombinant proteins for molecular biology, biochemistry, and cell biology experiments. Two forms of COS cell lines commonly used are COS-1 and COS-7. The COS cell line was obtained by immortalizing a cell line derived from kidney cells of the African green monkey with a version of the SV40 genome that can produce large T antigen but has a defect in genomic replication.
最佳转染条件:
Confluency of cell on the day of shaving 90%
GenJet? (μl) : DNA (μg) Ratio 3:1
Diluent for DNA and Transfection Reagent Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet?/DNA Complex 15 minutes at RT
Maximal Efficiency 48 hours
Transfection Results:
Reporter Gene EGFP
Plasmid pEGFP-N3 (CMV promoter)
Efficiency (GFP %) 88%
储存条件:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer. |