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产品名称:GenJet (Ver. II) DNA In Vitro Transfection Kit for Keratinocytes
功能:
GenJet™ DNA In Vitro Tranfection Reagent for keratinocytes is pre-optimized for transfecting human or mouse primary or immortalized keratinocytes. Keratinocytes are hard to transfect partly due to the transfection toxicity related cell death. In combination of our proprietary HapiCell™ Toxicity Removal Cocktail, the pre-optimized transfection reagent gave up to 50% efficiency with minimal cell death.
组份:
1x1.0 ml of GenJet™ DNA Transfection Reagent
1x1.0 ml (x50) of HapiCell™ Transfection Toxicity Removal Cocktail
最佳转染条件:
Confluency of cell on the day of shaving 90%
GenJet™ (µl) : DNA (µg) Ratio 3:1
Diluent for DNA and Transfection Reagent Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet™/DNA Complex 15 minutes at RT
Maximal Efficiency 48 hours
Transfection Results:
Reporter Gene EGFP
Plasmid pEGFP-N3 (CMV promoter)
Efficiency (GFP %) 56%
储存条件:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer. |