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产品名称:GenJet (Ver. II) DNA In Vitro Transfection Reagent for B16-F10 Cells
功能:
GenJet™ DNA In Vitro Tranfection Reagent for B16-F10 cell is pre-optimized for transfecting B16-F10 cells. B16-F10 is a derived murine melanoma cell line, which is well-established model for the study of experimental cancer therapies. Melanomas express different tumor-associated antigens, which are potential targets for novel designs of therapeutic cancer vaccines. Melanoma-associated antigens include tyrosinase and tyrosine-related protein (TRP)-1/2 or the MAGE and BAGE class of antigens. Many immunotherapeutic protocols have been tested using the murine B16-F10 melanoma cell line that originated in the syngeneic C57BL/6 (H-2b) mouse strain.
最佳转染条件:
Confluency of cell on the day of shaving 90%
GenJet™ (µl) : DNA (µg) Ratio 3:1
Diluent for DNA and Transfection Reagent Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet™/DNA Complex 15 minutes at RT
Maximal Efficiency 48 hours
Transfection Results:
Reporter Gene EGFP
Plasmid pEGFP-N3 (CMV promoter)
Efficiency (GFP %) 88%
储存条件:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.
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