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产品名称:GenJet (Ver. II) DNA In Vitro Transfection Reagent for M-PAC Cell
功能:
GenJet™ DNA In Vitro Tranfection Reagent for M-PAC is pre-optimized for transfecting M-PAC (also celled mPAC) cells. M-PAC is moderately impregnated pancreas acinar unit cells, also one of hard-to-transfect cell line.
Refer to the following optimal transfection conditions for maximal transfection efficiency on M-PAC cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.
最佳转染条件:
Confluency of cell on the day of shaving 90%
GenJet™ (µl) : DNA (µg) Ratio 3:1
Diluent for DNA and Transfection Reagent Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet™/DNA Complex 15 minutes at RT
Maximal Efficiency 48 hours
Transfection Results:
Reporter Gene EGFP
Plasmid pEGFP-N3 (CMV promoter)
Efficiency (GFP %) 60%
储存条件:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer. |