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产品名称:GenJet (Ver. II) DNA In Vitro Transfection Reagent for CV-1 Cell
功能:
GenJet™ DNA In Vitro Tranfection Reagent for CV-1 is pre-optimized for transfecting CV-1 cells. The CV-1 cell line was initiated in March of 1964 with a tissue section excised from the kidney of an adult male African green monkey (Cercopithecus aethiops). The fibroblast line was originally utilized in research focusing on the transformation of the cancer-causing Rous sarcoma virus (RSV), but now is popular as a host for acquired immunodeficiency disease (AIDS) research, as well as transfection experiments with simian virus 40 (SV40) and recombinant plasmid vectors. CV-1 cells exhibit fibroblast morphology, grow adherently to glass or plastic surfaces, and are negative for reverse transcriptase. The cells are known to be susceptible to several viruses, including poliovirus 1, herpes simplex, simian virus 40, California encephalitis, and both Eastern and Western equine encephalitis. The cells exhibit rapid growth and chromosome number shifts have been reported to occur at high passage levels.
最佳转染条件:
Confluency of cell on the day of shaving 80%
GenJet™ (µl) : DNA (µg) Ratio 3:1
Diluent for DNA and Transfection Reagent Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet™/DNA Complex 15 minutes at RT
Maximal Efficiency 36 hours
Transfection Results:
Reporter Gene EGFP
Plasmid pEGFP-N3 (CMV promoter)
Efficiency (GFP %) 75%
储存条件:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer. |