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Wortmannin
PI3K inhibitor - Autophagy Inhibitor
Wortmannin is a cell-permeable, fungal metabolite that acts as a potent, selective and irreversible inhibitor of phosphatidylinositol 3-kinase (PI3K) [1].
There is increasing evidence of the involvement of PI3K in Toll-like receptor (TLR) signaling [2]. Inhibition of PI3K with wortmannin enhances TLR-mediated inducible nitric-oxide synthase (iNOS) expression, activates NF-κB and up-regulates cytokine mRNA production [3].
Furthermore, PI3K is required for autophagy [4]. Autophagy is a complex pathway in which cell material can be sequestered and delivered to the lysosome for degradation. Inhibition of PI3K with wortmannin can inhibit autophagic sequestration [4].
Working concentration:
• 0.1-2.5 μM when used as an inhibitor in the TLR signaling cascade
• 0.1-10 μM when used as an inhibitor of autophagosome formation
Purity: >99% (HPLC)
Endotoxin level: <0.125 EU/mg
Solubility: 10 mM in DMSO or methanol
CAS number: 19545-26-7
Synonym: KY 12420
Molecular weight: 428.4
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