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THP1-XBlue™-MD2-CD14 Cells
THP1-XBlue™-MD2-CD14 cells derive from the THP1-XBlue™ cell line. THP1-XBlue™-MD2-CD14 cells stably express the human MD2 and CD14 genes. MD2 is an accessory molecule essential for LPS-induced TLR4 response. CD14, a macrophage-specific differentiation antigen, interacts with several TLRs, including TLR4 and TLR2. Overexpression of CD14 was found to increase the response to the majority of TLR ligands.
THP1-XBlue™-MD2-CD14 cells express an NF-κB/AP-1-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene. TLR stimulation in THP1-XBlue™-MD2-CD14 cells induces signaling cascades leading to the activation of NF-κB and AP-1 and the subsequent production of SEAP. The reporter protein is easily detectable and measurable when using QUANTI-Blue™, a medium that turns purple/blue in the presence of SEAP.
Antibiotic resistance: G418, Zeocin™
Growth medium: RPMI, 2-4 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice |