THP1-XBlue?-defMyD cells

THP1-XBlue™-defMyD Cells
THP1-XBlue™-defMyD cells are THP1-XBlue™ cells deficient in MyD88 activity. MyD88 is an adaptor protein involved in many signaling pathways. THP1-XBlue™-defMyD cells are unable to respond to the activation of receptors which signal through MyD88, such as TLR2, TLR4 and IL-1Rs. They remain responsive to MyD88-independent receptors, such as NOD1 and TNFR.
THP1-XBlue™-defMyD cells express an NF-κB/AP-1-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene. Activation of MyD88-independent signaling pathways in THP1-XBlue™-defMyD cells triggers the activation of NF-κB and AP-1 and the subsequent production of SEAP. The reporter protein is easily detectable and measurable when using QUANTI-Blue™, a medium that turns purple/blue in the presence of SEAP.
Antibiotic resistance: Hygromycin B, Zeocin™
Growth medium: RPMI, 2-4 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice