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THP1-XBlue™ Cells
THP1-XBlue™ cells derive from the human monocytic THP-1 cell line. THP1-XBlue™ cells stably express an NF-κB- and AP-1-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene.
Upon TLR stimulation, THP1-XBlue™ cells induce the activation of NF-κB and AP-1 and subsequently the secretion of SEAP. The reporter protein is easily detectable and measurable when using QUANTI-Blue™, a medium that turns purple/blue in the presence of SEAP.
Antibiotic resistance: Zeocin™
Growth medium: RPMI, 2-4 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice |