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THP1-defNLRP3 cells
Human monocytes with reduced NLRP3 activity
THP1-defNLRP3 cells are derived from THP-1 human monocytic cells. THP-1 cells are the most commonly used model cell line for the study of inflammasome activation as they express high levels of NLRP3, ASC and pro-caspase-1.
THP1-defNLRP3 cells have reduced NLRP3 activity but are proficient for ASC and caspase-1 activities.
THP1-defNLRP3 cells display significantly weaker responses to inducers of the NLRP3 inflammasome, such as ATP and MSU, when compared to THP1-Null cells. THP1-defNLRP3 cells also show a greatly reduced response to extracellular calcium, a danger signal that activates the NLRP3 inflammasome.
However, THP1-defNLRP3 cells may respond to signals that activate other ASC-dependent inflammasomes, such as NLRP1 and NLRC4 inflammasomes. THP1-defNLRP3 cells are designed as tools to study the involvement of NRLP3 in response to a given signal.
The positive control cell line for inflammasome studies are THP1-Null cells.
Antibiotic resistance: hygromycin B
Growth Medium: RPMI 1640 (2 mM L-glutamine, 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate) with 10% heat-inactivated fetal bovine serum, 100 μg/ml Normocin™, Pen-Strep (50 U/ml-50 μg/ml)
Guaranteed mycoplasma-free
Shipped on dry ice |