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THP1-Null cells
Positive control cell line for inflammasome studies
THP1-Null cells are derived from THP-1 human monocytic cells, that represent the most commonly used model cell line for the study of inflammasome activation as they express high levels of NLRP3, ASC and pro-caspase-1.
THP1-Null cells produce IL-1β upon stimulation with inflammasome inducers, such as MSU crystals and ATP.
As THP1-Null cells are fully efficient for NLRP3 and ASC activities, they are the positive control cell line for THP1-defASC and THP1-defNLRP3, that are deficient in ASC and NLRP3 respectively.
Antibiotic resistance: hygromycin B
Growth Medium: RPMI 1640 (2 mM L-glutamine, 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate) with 10% heat-inactivated fetal bovine serum, 100 μg/ml Normocin™, Pen-Strep (50 U/ml-50 μg/ml)
Guaranteed mycoplasma-free
Shipped on dry ice |