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别名：植物血凝；PHA，植物血球凝聚集素
外观：白色干燥粉末
特性：溶解性：溶于水和生理盐水，不溶于有机溶剂。
用途：细胞体外培养，细胞免疫功能的测定和染色体的研究等。
Sigma相关产品资料：
L2646 Sigma Lectin from Phaseolus vulgaris (red kidney bean)
Phytohemagglutinin PHA-M, lyophilized powder
Synonym: Phaseolus vulgaris agglutinin, PHA
EC Number: 232-718-7
Description
Analysis Note Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C.
Biochem/physiol Actions PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.1,2
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides.3 PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin.4 PHA-M is the mucoprotein form.4 Conjugates are prepared from the corresponding purified lectins.
Properties
form lyophilized powder
potency <40 μg per mL agglutination activity
composition Protein, 60-80% biuret
total impurities salt, free
storage temp. 2-8°C
Safety
Safety Statements 22-24/25
RTECS TJ3470000 |