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说明：生化研究，可用于有丝分裂的研究。
别名：Phaseolus vulgaris agglutinin；Lectin；PHA
特性：来源：菜豆
效价：<62.5 μg /mL 凝集活性<10 μg/mL促有丝分裂活性
Sigma相关产品资料：
Sigma L8754 Lectin from Phaseolus vulgaris (red kidney bean)
Phytohemagglutinin PHA-P, lyophilized powder
Description
Analysis Note Agglutination activity is expressed in mg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures.
Biochem/physiol Actions PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.1,2
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides.3 PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin.4 PHA-M is the mucoprotein form.4 Conjugates are prepared from the corresponding purified lectins.
Properties
form lyophilized powder
potency <62.5 μg per mL agglutination activity
<10 μg/mL mitogenic activity
total impurities salt, free
storage temp. 2-8°C
Safety
Safety Statements 22-24/25
RTECS TJ3470000 |