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ChIPAb+ EZH2, clone AC22 - ChIP Validated Antibody and Primer Set
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REFERENCES
The ablation of EZH2 uncovers its crucial role in rhabdomyosarcoma formation.
Marchesi, Irene, et al. (2012) Cell Cycle, 11: 3828-36 (2012)
Polycomb protein EZH2 regulates tumor invasion via the transcriptional repression of the metastasis suppressor RKIP in breast and prostate cancer.
Gang Ren,Stavroula Baritaki,Himangi Marathe,Jingwei Feng,Sungdae Park,Sandy Beach,Peter S Bazeley,Anwar B Beshir,Gabriel Fenteany,Rohit Mehra,Stephanie Daignault,Fahd Al-Mulla,Evan Keller,Ben Bonavida,Ivana de la Serna,Kam C Yeung (2012) Cancer research.72
Long noncoding RNA high expression in hepatocellular carcinoma facilitates tumor growth through enhancer of zeste homolog 2 in humans.
Fu Yang,Ling Zhang,Xi-song Huo,Ji-hang Yuan,Dan Xu,Sheng-xian Yuan,Nan Zhu,Wei-ping Zhou,Guang-shun Yang,Yu-zhao Wang,Jing-li Shang,Chun-fang Gao,Feng-rui Zhang,Fang Wang,Shu-han Sun (2011) Hepatology (Baltimore, Md.).54
Coordinated regulation of transcriptional repression by the RBP2 H3K4 demethylase and Polycomb-Repressive Complex 2.
Pasini, Diego, et al. (2008) Genes Dev., 22: 1345-55 (2008)
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Species Reactivity Key Applications Host Format Antibody Type
H, M, Vrt, R IP, ChIP-seq Mouse null Monoclonal Antibody
Description:
ChIPAb+ EZH2, clone AC22
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Trade Name:
Upstate (Millipore)
Product Overview:
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ EZH2, clone AC22 antibody/primer set includes the EZH2, clone AC22 antibody, a negative control antibody (purified mouse IgG), and qPCR primers which amplify a 135 bp region within the promoter of the human MYT-1 gene. The EZH2, clone AC22 antibody and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of EZH2-associated chromatin.
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Specificity:
Recognizes EZH2, MW: ~85-100 kDa.
Molecular Weight:
~85 kDa
Epitope:
a.a. 353-451
Immunogen:
EZH2, clone AC22 purified antibody is made against a GST fusion protein corresponding to amino acids 353-451 of human EZH2.
Isotype:
IgG2a
Species Reactivity:
Human
Mouse
Vertebrates
Rat
Species Reactivity Note:
Human, mouse, rat. Not tested in other species. The immunogen sequence is identical in a wide range of animal and plant species, so broad cross-reactivity is expected.
Application Notes:
Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from HeLa S3 cells (4 X 106 cell equivalents per IP) was subjected to chromatin immunoprecipitation using 1 µg of either a normal mouse IgG or Anti-EZH2, clone AC22 antibody and the Magna ChIP G Kit (Cat. # 17-611).
Successful immunoprecipitation of EZH2-associated DNA fragments was verified by qPCR using GAPDH promoter (negative) and MYT-1 promoter (positive) primers (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna G ChIP™ (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental d |