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ChIPAb+ SUZ12 - ChIP Validated Antibody and Primer Set
Species Reactivity Key Applications Host Format Antibody Type
H, M, R, Vrt IF, IP Mouse null Monoclonal Antibody
Description:
ChIPAb+ SUZ12
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Trade Name:
Upstate (Millipore)
Product Overview:
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ SUZ12, set includes the polycomb protein SUZ12 antibody (also known as suppressor of zeste 12 protein homolog or chromatin precipitated E2F target 9 protein), a negative control antibody (purified mouse IgG), and qPCR primers which amplify a 138 bp region upstream of human HoxA2 gene. The SUZ12 and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of SUZ12 associated
chromatin.
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Specificity:
Recognizes SUZ12, Mr 95 kDa.
Molecular Weight:
~95 kDa
Immunogen:
SUZ12, purified antibody is made against human SUZ12.
Isotype:
IgG1κ
Species Reactivity:
Human
Mouse
Rat
Vertebrates
Species Reactivity Note:
Human, mouse, rat. Not tested in other species.
Application Notes:
Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from Ntera2 cells (3 X 106 cell equivalents per IP) was subjected to chromatin immunoprecipitation using 1 µg of either a normal mouse IgG or Anti-SUZ12 antibody and the Magna ChIP G Kit (Cat. # 17-611).
Successful immunoprecipitation of SUZ12 associated DNA fragments was verified by qPCR using GAPDH promoter (negative) and HoxA2 (positive) Primers (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna G ChIP™ (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
Western Blot Analysis:
Representative lot data.
Lysates from HeLa cells were resolved by electrophoresis, transferred to PVDF and probed with anti-SUZ12 (1:1,000 dilution). Proteins were visualized using goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection (Please see figures).
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Control:
Includes negative control mouse IgG antibody and primers specific for human HoxA2 upstream region.
Quality Assurance:
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from Ntera2 cells (3 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either a normal mouse IgG or Anti-SUZ12 antibody and the Magna ChIP™ G Kit (Cat. # 17-611).
Successful immunoprecipitation of SUZ12 associated DNA fragments was verified by qPCR using Control Primers (Please see figures).
Please refer to the EZ-Magna ChIP™ G (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
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Presentation:
Anti-SUZ12 (mouse monoclonal IgG). One vial containing 50 μg of protein A purified antibody in PBS containing 0.05% sodium azide. May contain 30% glycerol (see certificate of analysis). Store at -20°C.
Normal Mouse IgG. Two vials containing 25 μg of pu |