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ChIPAb+ EED - ChIP Validated Antibody and Primer Set
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REFERENCES
Corepressor protein CDYL functions as a molecular bridge between polycomb repressor complex 2 and repressive chromatin mark trimethylated histone lysine 27.
Yu Zhang,Xiaohan Yang,Bin Gui,Guojia Xie,Di Zhang,Yongfeng Shang,Jing Liang (2011) The Journal of biological chemistry.286
The polycomb group protein Suz12 is required for embryonic stem cell differentiation.
Pasini, Diego, et al. (2007) Mol. Cell. Biol., 27: 3769-79 (2007)
The Polycomb group proteins bind throughout the INK4A-ARF locus and are disassociated in senescent cells.
Bracken, Adrian P, et al. (2007) Genes Dev., 21: 525-30 (2007)
Genome-wide mapping of Polycomb target genes unravels their roles in cell fate transitions.
Bracken, Adrian P, et al. (2006) Genes Dev., 20: 1123-36 (2006)
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Species Reactivity Key Applications Host Format Antibody Type
H, M WB, IP Mouse null Monoclonal Antibody
Description:
ChIPAb+ EED
Promotional Text:
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Trade Name:
Upstate (Millipore)
Product Overview:
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ EED set includes the polycomb protein EED antibody, a negative control antibody (purified mouse IgG), and qPCR primers which amplify a 138 bp region upstream of human HoxA2 gene. The EED and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of EED associated chromatin.
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Specificity:
Recognizes EED, MW: ~50 kDa.
Molecular Weight:
~50 kDa
Immunogen:
Recombinant fusion protein of full length murine EED tagged with MBP.
Isotype:
IgG2aκ
Species Reactivity:
Human
Mouse
Species Reactivity Note:
Human, Mouse.
Not tested in other species.
Application Notes:
Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from Ntera2 cells (3 X 106 cell equivalents per IP) was subjected to chromatin immunoprecipitation using 1 µg of either a normal mouse IgG, or Anti-EED antibody and the Magna ChIP G Kit (Cat. # 17-611).
Successful immunoprecipitation of EED associated DNA fragments was verified by qPCR using GAPDH promoter (negative) and HoxA2 (positive) Primers (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna G ChIP™ (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
Western Blot Analysis:
Representative lot data.
Lysates from A431 cell lysate were resolved by electrophoresis, transferred to PVDF and probed with anti-EED. Proteins were visualized using goat anti-mouse secondary antibody conjugated to HRP and chemiluminescence detection (Please see figures).
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Control:
Includes negative control mouse IgG antibody and primers specific for human HoxA2 upstream region.
Quality Assurance:
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from Ntera2 cells (3 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 &m |