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ChIPAb+ Dimethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer Set
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REFERENCES
Zinc-induced Dnmt1 expression involves antagonism between MTF-1 and nuclear receptor SHP.
Yuxia Zhang,Glen K Andrews,Li Wang (2012) Nucleic acids research.40
Nuclear receptor SHP inhibition of Dnmt1 expression via ERRγ.
Yuxia Zhang,Li Wang (2011) FEBS letters.585
The miR-124-Sox9 paramutation: RNA-mediated epigenetic control of embryonic and adult growth.
Valérie Grandjean,Pierre Gounon,Nicole Wagner,Luc Martin,Kay D Wagner,Florence Bernex,François Cuzin,Minoo Rassoulzadegan (2009) Development (Cambridge, England).136
cAMP Prevents Glucose-Mediated Modifications of Histone H3 and Recruitment of the RNA Polymerase II Holoenzyme to the L-PK Gene Promoter.
Burke, Susan J, et al. (2009) J. Mol. Biol., (2009)
Species Reactivity Key Applications Host Format Antibody Type
H, M, Ma WB, IP, ChIP Rabbit Serum Polyclonal Antibody
Description:
ChIPAb+ Dimethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer Set
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Trade Name:
Upstate (Millipore)
Product Overview:
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ Dimethyl-Histone H3 (Lys9) set includes the anti-dimethyl-histone H3 (Lys9) antibody, a negative control antibody (normal rabbit serum), and qPCR primers which amplify a 110 bp region within the promoter of the human β-globin gene. The dimethyl-histone H3 (Lys9) and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of dimethyl-histone H3 (Lys9) associated chromatin.
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Specificity:
Dimethyl-Histone H3 (Lys9)
Molecular Weight:
17 kDa
Epitope:
Dimethyl Lys9
Immunogen:
The dimethyl-histone H3 (Lys9) rabbit serum is made against a KLH-conjugated, branched synthetic peptide containing the sequence ..Rme2KSTG.., in which me2K corresponds to dimethyl-lysine at residue 9 of human histone H3
Species Reactivity:
Human
Mouse
Mammals
Application Notes:
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from untreated HeLa cells (1 X 106 cell equivalents) was subjected to chromatin Immunoprecipitation using 4 μL of either a normal rabbit antiserum or Antidimethyl-Histone H3 (Lys9) serum and the Magna ChIP A (Cat. #17-610) Kit (Please see figures). Successful Immunoprecipitation of dimethyl-histone H3 (Lys9) associated DNA fragments was verified by qPCR using β-globin ChIP Primers flanking the human β-globin promoter or primers amplifying the promoter of human GAPDH, which is transcriptionally inactive in HeLa cells. Percent Input relative to standard curves for each qPCR primer set are shown.
Please refer to the EZ-Magna A ChIP™ (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
Western blot analysis and peptide inhibition:
HeLa Acid extract were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-dimethyl-Histone H3 (Lys9) (1:500, Lane 1) or preincubated with 0.4 μM Histone H3 peptide with following modifications:
Lane 2: Linear non-modified
Lane 3: |