ChIPAb+ Ubiquityl-Histone H2B - ChIP Validated Antibody and Primer Set
Close
REFERENCES
H2B monoubiquitylation is a 5'-enriched active transcription mark and correlates with exon-intron structure in human cells.
Inkyung Jung,Seung-Kyoon Kim,Mirang Kim,Yong-Mahn Han,Yong Sung Kim,Dongsup Kim,Daeyoup Lee (2012) Genome research.22
Species Reactivity Key Applications Host Format Antibody Type
M, H, Vrt, R, Pl WB, IF, ChIP Mouse Protein G Purified Monoclonal Antibody
Description:
ChIPAb+ Ubiquityl-Histone H2B - ChIP Validated Antibody and Primer Set
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Product Overview:
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ Ubiquityl-Histone H2B, (clone 56) set includes the Ubiquityl-Histone H2B, (clone 56) antibody, a negative control antibody (purified Mouse IgG), and qPCR primers which amplify a 213 bp region within the coding region of the human GAPDH gene.
The ubiquityl-Histone H2B and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Ubiquityl-Histone H2B associated chromatin.
View All »
Specificity:
Recognizes histone H2B ubiquitinated on lysine 120 but not free ubiquitin or histone H2B in its unmodified state.
Molecular Weight:
~25 kDa
Epitope:
Lys120 ubiquitination site
Immunogen:
Immunogen used was a branched peptide corresponding to the ubiquitination site on human histone H2B.
Modifications:
Ubiquitylation
Isotype:
IgG2aκ
Species Reactivity:
Mouse
Human
Vertebrates
Rat
Green Plants
View All »
Species Reactivity Note:
Human, mouse, rat. Not tested in other species. The immunogen sequence is identical in a wide range of animal and plant species, so broad cross-reactivity is expected.
Application Notes:
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa S3 cells (1 X 106 cell equivalents per IP) was subjected to chromatin immunoprecipitation using 1 μg of either a normal mouse IgG or Anti-Ubiquityl-Histone H2B antibody and the Magna ChIP G Kit (Cat. # 17-611).
Successful immunoprecipitation of ubiquityl-Histone H2B associated DNA fragments was verified by qPCR using GAPDH promoter (negative) and GAPDH coding (positive) Primers (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna G ChIP™ (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
Western Blot Analysis:
HeLa cell lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-Ubiquityl-histone H2B at a dilution of 1:4000 (Please see figures).
View All »
Control:
Includes negative control mouse IgG antibody and primers specific for human GAPDH coding region.
Quality Assurance:
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa S3 cells (1X 106 cell equivalents per IP) were subjected to chromatin immuno-precipitation using 1 µg of either a normal mouse IgG or A |