|
ChIPAb+ HP1γ - ChIP Validated Antibody and Primer Set
Close
REFERENCES
Evidence for the existence of an HP1-mediated subcode within the histone code.
Lomberk, Gwen, et al. (2006) Nat. Cell Biol., 8: 407-15 (2006)
Phosphorylation site mutations in heterochromatin protein 1 (HP1) reduce or eliminate silencing activity.
Zhao, T, et al. (2001) J. Biol. Chem., 276: 9512-8 (2001)
The HP1 protein family: getting a grip on chromatin.
Eissenberg, J C and Elgin, S C (2000) Curr. Opin. Genet. Dev., 10: 204-10 (2000)
Species Reactivity Key Applications Host Format Antibody Type
H, M, Vrt Cell Function Assay, WB, ChIP Mouse Ascites Monoclonal Antibody
Description:
ChIPAb+ HP1γ - ChIP Validated Antibody and Primer Set
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Product Overview:
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ HP1γ antibody/primer set includes the HP1g antibody, a negative control antibody (purified mouse IgG), and qPCR primers which amplifies a 213 bp region within the coding region of the human GAPDH gene. The HP1γ and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of HP1γ associated chromatin.
View All »
Specificity:
Recognizes HP1γ, MW: ~25 kDa.
Molecular Weight:
~25 kDa
Epitope:
a.a. 17-173
Immunogen:
HP1γ ascites is made against a GST fusion protein corresponding to amino acids 17-173 of human HP1γ.
Isotype:
IgG1
Species Reactivity:
Human
Mouse
Vertebrates
Species Reactivity Note:
Human, mouse. Not tested in other species. The immunogen sequence is identical in a wide range of animal and plant species, so broad cross-reactivity is expected.
Application Notes:
Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from HeLa S3 cells (1 X 106 cell equivalents per IP) was subjected to chromatin immunoprecipitation using 5 µg of either a normal mouse IgG or HP1γ antibody and the Magna ChIP G Kit (Cat. # 17-611).
Successful immunoprecipitation of HP1γ associated DNA fragments was verified by qPCR using MYOD promoter (negative) and GAPDH coding (positive) (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna G ChIP™ (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
View All »
Control:
Includes negative control mouse IgG antibody and primers specific for of human GAPDH coding region.
Quality Assurance:
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa S3 cells (1 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 5 µg of either a normal mouse IgG or HP1γ antibody and the Magna ChIP™ G Kit (Cat. # 17-611). Successful immunoprecipitation of HP1γ-associated DNA fragments was verified by qPCR using Control Primers (Please see figures).
Please refer to the EZ-Magna ChIP&tra |