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ChIPAb+ Trimethyl-Histone H3 (Lys9)
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REFERENCES
Cutting edge: an inactive chromatin configuration at the IL-10 locus in human neutrophils.
Tamassia, Nicola, et al. (2013) J. Immunol., 190: 1921-5 (2013)
Sex-specific dynamics of global chromatin changes in fetal mouse germ cells.
Masanobu Abe,Shirley Y Tsai,Seung-Gi Jin,Gerd P Pfeifer,Piroska E Szabó (2011) PloS one.6
Long intronic GAA*TTC repeats induce epigenetic changes and reporter gene silencing in a molecular model of Friedreich ataxia.
E Soragni,D Herman,S Y R Dent,J M Gottesfeld,R D Wells,M Napierala (2008) Nucleic acids research.36
The many faces of histone lysine methylation.
Lachner, Monika and Jenuwein, Thomas (2002) Curr. Opin. Cell Biol., 14: 286-98 (2002)
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Species Reactivity Key Applications Host Format Antibody Type
Vrt WB, DB, ChIP Rabbit Purified Polyclonal Antibody
Description:
ChIPAb+ Trimethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer Set
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Trade Name:
Upstate (Millipore)
Product Overview:
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ Trimethyl-Histone H3 (Lys9) set includes the Anti-trimethyl-Histone H3 (Lys9) antibody, a negative control antibody (purified rabbit IgG), and qPCR primers which amplify a 117 bp region within the 3’ end of the human ZNF554 gene. The trimethyl-histone H3 (Lys9) and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of trimethyl-histone H3 (Lys9) associated chromatin.
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Specificity:
Recognizes histone H3, Mr 17 kDa, trimethylated at lysine 9.
Molecular Weight:
17kDa
Epitope:
Trimethyl Lys9
Immunogen:
The trimethyl-histone H3 (Lys9) purified antibody is made against BSA-conjugated, synthetic peptide containing the sequence …AR[me3K]S… in which me3K corresponds to trimethyl lysine 9 of human Histone H3.
Species Reactivity:
Vertebrates
Species Reactivity Note:
Human, mouse. The immunogen sequence is identical in a wide range of animal and plant species, so broad cross-reactivity is expected.
Application Notes:
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from 2x106 HeLa cells were subjected to chromatin immunoprecipitation using 4 μg purified antibody or normal rabbit IgG and the Magna ChIP™ A kit (Cat. # 17-610).
Successful enrichment of trimethyl-Histone H3 (Lys9) associated DNA fragments was verified by qPCR using ChIP Primers GAPDH flanking the human GAPDH promoter and primers targeting the promoter of human MyoD.
Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) kit protocols for experimental details.
Western Blot Analysis:
Recombinant Histone H3 (Lane 1) and HeLa acid extract (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-trimethyl-Histone H3 (Lys9), (1 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).
Beadlyte® Histone Peptide Specificity Assay:
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