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ChIPAb+ Trimethyl-Histone H3 (Lys27) - ChIP Validated Antibody and Primer Set
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REFERENCES
Cutting edge: an inactive chromatin configuration at the IL-10 locus in human neutrophils.
Tamassia, Nicola, et al. (2013) J. Immunol., 190: 1921-5 (2013)
Maternal low-protein diet causes epigenetic deregulation of HMGCR and CYP7α1 in the liver of weaning piglets.
Rihua Cong,Yimin Jia,Runsheng Li,Yingdong Ni,Xiaojing Yang,Qinwei Sun,Nahid Parvizi,Ruqian Zhao (2012) The Journal of nutritional biochemistry.23
SINE Retrotransposons Cause Epigenetic Reprogramming of Adjacent Gene Promoters.
Marcos R H Estécio,Juan Gallegos,Mhair Dekmezian,Yue Lu,Shoudan Liang,Jean-Pierre J Issa (2012) Molecular cancer research : MCR.10
Telomere length regulates TERRA levels through increased trimethylation of telomeric H3K9 and HP1α.
Arnoult, Nausica, et al. (2012) Nat. Struct. Mol. Biol., 19: 948-56 (2012)
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Species Reactivity Key Applications Host Format Antibody Type
H, M WB, ChIP Rabbit Protein A Purified Polyclonal Antibody
Description:
ChIPAb+ Trimethyl-Histone H3 (Lys27) - ChIP Validated Antibody and Primer Set
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Trade Name:
Upstate (Millipore)
Product Overview:
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot,
every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context.
Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ Trimethyl-Histone H3 (Lys27) set includes the Anti-Trimethyl-Histone H3 (Lys27) antibody, a negative control antibody (purified rabbit IgG), and qPCR primers which amplify a 139 bp region of human alpha-Satellite. The Trimethyl-Histone H3 (Lys27) and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Trimethyl-Histone H3 (Lys27)-associated chromatin.
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Specificity:
trimethyl-Histone H3 (Lys27)
Molecular Weight:
17kDa
Epitope:
Trimethyl Lys27
Immunogen:
KLH-conjugated, synthetic 2X-branched peptide containing the sequence ...AR(me3K)SAP... in which me3K corresponds to trimethyl-lysine at residue 27 of human Histone H3.
Species Reactivity:
Human
Mouse
Species Reactivity Note:
Broad species cross-reactivity expected.
Application Notes:
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from 2x106 HeLa cells were subjected to chromatin immunoprecipitation using 4 μg purified anti-trimethyl-Histone H3 (Lys27) antibody or normal rabbit IgG and the Magna ChIP™ A kit (Cat. #17-610). Successful enrichment of trimethyl-Histone H3 (Lys27) associated DNA fragments was verified by qPCR using ChIP Primers GAPDH flanking the human GAPDH promoter and primers targeting the promoter of human MyoD.
Please refer to the EZ-Magna ChIP™ A (Cat. #17-408) or EZChIP™ (Cat. #17-371) kit protocols for experimental details.
Western Blot Analysis:
Recombinant Histone H3 (Lane 2) and HeLa cell acid extracts (Lane 1) were resolved by electrophoresis, transferred to nitrocellulose and probed with a 1:5000 dilution of anti- trimethyl histone H3 Lys27 (0.4 μg/mL).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).
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