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ChIPAb+ RNA Pol II - ChIP Validated Antibody and Primer Set
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REFERENCES
Discovery of SMAD4 promoters, transcription factor binding sites and deletions in juvenile polyposis patients.
Daniel Calva,Fadi S Dahdaleh,George Woodfield,Ronald J Weigel,Jennifer C Carr,Sathivel Chinnathambi,James R Howe (2011) Nucleic acids research.39
Species Reactivity Key Applications Host Format Antibody Type
H, M, Yeast (S. cerevisiae), R IF, IP, ChIP Mouse Purified Monoclonal Antibody
Description:
ChIPAb+ RNA Pol II - ChIP Validated Antibody and Primer Set
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Trade Name:
Upstate (Millipore)
Product Overview:
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ RNA Pol II set includes the RNA Pol II antibody, the negative control antibody (mouse IgG), and qPCR primers flanking the human GAPDH promoter, yielding a 166 bp product. The RNA Pol II and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of RNA Pol II associated chromatin.
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Specificity:
Recognizes RNA Pol II
Molecular Weight:
210-220 kDa
Immunogen:
The RNA Pol II antibody is made against a peptide corresponding to the C-terminal domain of RNA Pol II.
Isotype:
IgG1
Species Reactivity:
Human
Mouse
Yeast (S. cerevisiae)
Rat
Species Reactivity Note:
Proven to react with human, and mouse. Other species predicted to cross-react based upon sequence conservation are rat and yeast.
Application Notes:
Western Blot Analysis:
3T3 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-RNA polymerase II (0.1 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).
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Control:
Included negative control antibody mouse IgG and control primers specific for human GAPDH.
Quality Assurance:
Routinely evaluated by chromatin immunoprecipitation on HeLa nuclear extract.
Purification Method:
Protein G purfied
Presentation:
Anti-RNA Pol II (mouse monoclonal IgG1,purified). One vial containing 25 μg of purified antibody in 25 μL volume. Store at -20°C.
Normal Mouse IgG. One vial containing 25 ug of mouse IgG in 25 μL volume. Store at -20°C.
Control Primers p21. One vial containing 75 μL of 5 μM of each primer specific for a region of the human GAPDH promoter. Store at -20°C.
FOR: TAC TAG CGG TTT TAC GGG CG
REV: TCG AAC AGG AGG AGC AGA GAG
CGA
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Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
UniProt Number:
P24928
Entrez Gene Number:
NP_000928
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption |