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ChIPAb+ Acetyl-Histone H3 - ChIP Validated Antibody and Primer Set
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REFERENCES
Identification of STAT2 serine 287 as a novel regulatory phosphorylation site in type I interferon-induced cellular responses.
Steen, Håkan C, et al. (2012) J. Biol. Chem., (2012)
Dynamics of plant histone modifications in response to DNA damage.
Georgina E Drury,Adam A Dowle,David A Ashford,Wanda M Waterworth,Jerry Thomas,Christopher E West (2012) The Biochemical journal.445
Administration of vorinostat disrupts HIV-1 latency in patients on antiretroviral therapy.
N M Archin,A L Liberty,A D Kashuba,S K Choudhary,J D Kuruc,A M Crooks,D C Parker,E M Anderson,M F Kearney,M C Strain,D D Richman,M G Hudgens,R J Bosch,J M Coffin,J J Eron,D J Hazuda,D M Margolis (2012) Nature.487
Lysine-specific demethylase 1 (LSD1) and histone deacetylase 1 (HDAC1) synergistically repress proinflammatory cytokines and classical complement pathway components.
Andreas Janzer,Soyoung Lim,Florian Fronhoffs,Naima Niazy,Reinhard Buettner,Jutta Kirfel (2012) Biochemical and biophysical research communications.421
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Species Reactivity Key Applications Host Format Antibody Type
H, M, T WB, ChIP Rabbit Purified Polyclonal Antibody
Description:
ChIPAb+ Acetyl-Histone H3 - ChIP Validated Antibody and Primer Set
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Trade Name:
Upstate (Millipore)
Product Overview:
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ Acetyl-Histone H3 set contains purified rabbit polyclonal antibody and the normal rabbit IgG antibody, which can be used to demonstrate that the acetyl-histone H3 antibody is capable of precipitating acetyl-histone H3 associated chromatin. The qPCR primers included flank the human GAPDH promoter and produce a 166 base pair PCR product.
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Specificity:
Recognizes acetyl-histone H3
Molecular Weight:
17 kDa
Immunogen:
The acetyl-histone H3 antibody is made against a peptide corresponding to amino acids 1-20 of Tetrahymena histone H3 ARTKQTAR[K*]STGG[K*]APRKQL(C) where K* is acetylated)
Species Reactivity:
Human
Mouse
Tetrahymena
Species Reactivity Note:
Proven to react with human and mouse.
Tetrahymena predicted to cross-react based upon sequence of antibody generating peptide.
Application Notes:
Western Blot Analysis:
Acid-extracted proteins from HeLa cells treated with 5 mM sodium butyrate for 24 hours (Lane 2) and untreated HeLa cells (Lane 1) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-acetyl-Histone H3 (0.01 μg/mL).
Proteins were visualized using a goat-rabbit secondary antibody conjugated to HRP and a chemilumnescence detection system (Please see figures).
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Control:
Included negative control antibody rabbit IgG and control primers specific for human GAPDH promoter.
Quality Assurance:
Routinely evaluated by chromatin immunoprecipitation on HeLa nuclear extract.
Purification Method:
Protein A purfied
Presentation:
Anti-Acetyl-Histone H3 (rabbit polyclonal IgG). One vial containing 125 ug of protein A purified antibody in 125 μL of storage buffer containing 0.1 M |