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ChIPAb+ Trimethyl-Histone H3 (Lys4) - ChIP Validated Antibody and Primer Set, rabbit monoclonal
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REFERENCES
Cutting edge: an inactive chromatin configuration at the IL-10 locus in human neutrophils.
Tamassia, Nicola, et al. (2013) J. Immunol., 190: 1921-5 (2013)
Epigenetic histone modification of Epstein-Barr virus BZLF1 promoter during latency and reactivation in Raji cells.
Takayuki Murata,Yutaka Kondo,Atsuko Sugimoto,Daisuke Kawashima,Shinichi Saito,Hiroki Isomura,Teru Kanda,Tatsuya Tsurumi (2012) Journal of virology.86
The von Hippel-Lindau tumor suppressor protein regulates gene expression and tumor growth through histone demethylase JARID1C.
X Niu,T Zhang,L Liao,L Zhou,D J Lindner,M Zhou,B Rini,Q Yan,H Yang (2012) Oncogene.31
Extensive transcriptional regulation of chromatin modifiers during human neurodevelopment.
Matthias K Weng,Bastian Zimmer,Dominik Pöltl,Marc P Broeg,Violeta Ivanova,John A Gaspar,Agapios Sachinidis,Ullrich Wüllner,Tanja Waldmann,Marcel Leist (2012) PloS one.7
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Species Reactivity Key Applications Host Format Antibody Type
H, M, Ma WB, ChIP, ChIP-seq Rabbit Purified Monoclonal Antibody
Description:
ChIPAb+ Trimethyl-Histone H3 (Lys4) - ChIP Validated Antibody and Primer Set, rabbit monoclonal
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Trade Name:
Upstate (Millipore)
Product Overview:
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ Trimethyl-Histone H3 (Lys4) set includes the anti-trimethyl-histone H3 (Lys4) antibody, a negative control antibody (normal rabbit serum), and qPCR primers which amplify a 166 base pair region within the promoter of the human GAPDH gene. The trimethyl-histone H3 (Lys4) and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of trimethyl-histone H3 (Lys4)-associated chromatin.
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Specificity:
Trimethyl-Histone H3 (Lys4)
Molecular Weight:
17 kDa
Epitope:
Trimethyl Lys4
Immunogen:
The trimethyl-histone H3 (Lys4) antibody is made against a BSA-conjugated, synthetic peptide containing the sequence …RT[me3K]QT… in which me3K corresponds to trimethyl-lysine 4 of human histone H3
Isotype:
IgG
Species Reactivity:
Human
Mouse
Mammals
Application Notes:
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from untreated or UV treated (6 hrs, 50 joules/m2.) U2OS cells (3 X 106 cell equivalents per IP) was subjected to chromatin immunoprecipitation using 3 μL of rabbit Anti-Trimethyl Histone H3 (Lys4) and the Magna ChIP™ A (Cat. # 17-610) Kit. Immunoprecipitation of trimethyl histone H3 (Lys4) associated DNA fragments was verified by qPCR using ChIP Primers p21 flanking the human p21 promoter that contains an Sp1 binding site (Please see figures).
Fold Increase is a ratio of normalized mean IP quantities extracted from standard curves derived from inputs of each chromatin sample. Trimethyl-histone (Lys4) immunoprecipitable activity associated with this promoter increases with UV treatment as observed in other studies.
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