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ChIPAb+ Acetyl-Histone H4 - ChIP Validated Antibody and Primer Set
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REFERENCES
Biomechanical stimulation of osteoblast gene expression requires phosphorylation of the RUNX2 transcription factor.
Yan Li,Chunxi Ge,Jason P Long,Dana L Begun,Jose A Rodriguez,Steven A Goldstein,Renny T Franceschi (2012) Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.27
Species Reactivity Key Applications Host Format Antibody Type
H, Eu WB, Mplex, Cell Function Assay, ChIP Rabbit Serum Polyclonal Antibody
Description:
ChIPAb+ Acetyl-Histone H4 - ChIP Validated Antibody and Primer Set
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Trade Name:
Upstate (Millipore)
Product Overview:
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ Acetyl-Histone H4 set includes purified rabbit polyclonal antiserum and the normal rabbit IgG antiserum, which can be used to demonstrate that the acetyl-histone H4 antibody is capable of precipitating acetyl-histone H4 associated chromatin. The qPCR primers included flank the human GAPDH promoter and produce a 166 base pair PCR product.
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Specificity:
Recognizes acetylated histone H4 of approximately 10 kDa. Cross-reacts with acetylated histone H2B from Tetrahymena and weakly crossreacts with acetylated histone H2B from HeLa cells.
May cross-react with other acetylated proteins.
Molecular Weight:
10 kDa
Immunogen:
The acetyl-histone H4 antiserum is made against a peptide corresponding to amino acids 2-19 of Tetrahymena histone H4.
Species Reactivity:
Human
Eukaryote
Species Reactivity Note:
Proven to react with human samples. Predicted broad cross-reactivity among eukaryotes based upon sequence similarity.
Application Notes:
Western Blot Analysis:
Acidextracted proteins from normal HeLa cells (Lane 1) and HeLa cells treated with 5 mM sodium butyrate for 24 hours (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-acetyl Histone H4 (1:2000).
Proteins were visualized using a goat-anti rabbit secondary antibody conjugated to HRP and a chemi-luminescence detection system. (Please see figures).
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Control:
Included negative control rabbit IgG antiserum and control primers specific for human GAPDH promoter.
Quality Assurance:
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from untreated HeLa cells (1 X 106 cell equivalents) was subjected to chromatin immunoprecipitation using 4 μL of either a normal rabbit antiserum or Anti-Acetyl Histone
H4 serum and the Magna ChIP A (Cat. # 17-610) Kit. Successful immunoprecipitation of acetyl histone H4 associated DNA fragments was verified by qPCR using control ChIP Primers flanking the human GAPDH promoter (Please see figures).
Please refer to the EZ-Magna A ChIP™ (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
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Presentation:
Anti-Acetyl-Histone H4 (rabbit polyclonal IgG). One vial containing 100 μL of antiserum containing 0.05% sodium azide. Store at -20°C.
Normal Rabbit Serum. One vial containing 100 uL antiserum containing 0.05% |