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Anti-phospho-BRCA1 (Ser1497) Antibody
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REFERENCES
BRCA1 is a cell cycle-regulated nuclear phosphoprotein.
Ruffner, H and Verma, I M (1997) Proc. Natl. Acad. Sci. U.S.A., 94: 7138-43 (1997)
Species Reactivity Key Applications Host Format Antibody Type
H WB Rabbit Purified Polyclonal Antibody
Description:
Anti-phospho-BRCA1 (Ser1497) Antibody
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Trade Name:
Upstate (Millipore)
Specificity:
phosphorylated BRCA1
Molecular Weight:
250kDa
Immunogen:
KLH-conjugated, synthetic phospho-peptide corresponding to residues 1489-1500 of human BRCA1 (C-KEPGVERS[pS]PSK)
Modifications:
Phosphorylation
Isotype:
IgG
Species Reactivity:
Human
Quality Assurance:
routinely evaluated by immunoblot on HeLa Nuclear Extract
Purification Method:
Protein A chromatography
Presentation:
of 0.1M Tris glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of 30% glycerol
Storage Conditions:
2 years at -20°C
UniProt Number:
P38398
Entrez Gene Number:
NM_007294
Gene Symbol:
BRCA1
RNF53
BRCAI
PSCP
BRCC1
IRIS
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
Western Blotting
Entrez Gene Summary:
This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC for BRCA1-associated genome surveillance complex. This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complex. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants have been described for this gene but only some have had their full-length natures identified.
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UniProt Summary:
FUNCTION: SwissProt: P38398 # Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Mediates E2-dependent ubiquitination. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation.
SIZE: 1863 amino acids; 207721 Da
SUBUNIT: Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBN protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. CTIP interacts specifically with the BRCT domains. Interacts with RNA polyme |