|
Anti-MeCP2 Antibody
Close
REFERENCES
Disease Modeling Using Embryonic Stem Cells: MeCP2 Regulates Nuclear Size and RNA Synthesis in Neurons.
Morteza Yazdani,Rub Deogracias,Jacky Guy,Raymond A Poot,Adrian Bird,Yves-Alain Barde (2012) Stem cells (Dayton, Ohio).30
Nuclear calcium signaling controls methyl-CpG-binding protein 2 (MeCP2) phosphorylation on serine 421 following synaptic activity.
Buchthal, Bettina, et al. (2012) J. Biol. Chem., 287: 30967-74 (2012)
Novel MeCP2 isoform-specific antibody reveals the endogenous MeCP2E1 expression in murine brain, primary neurons and astrocytes.
Zachariah, Robby M, et al. (2012) PLoS ONE, 7: e49763 (2012)
The stress oncoprotein LEDGF/p75 interacts with the methyl CpG binding protein MeCP2 and influences its transcriptional activity.
Lai Sum Leoh,Bart van Heertum,Jan De Rijck,Maria Filippova,Leslimar Rios-Colon,Anamika Basu,Shannalee R Martinez,Sandy S Tungteakkhun,Valeri Filippov,Frauke Christ,Marino De Leon,Zeger Debyser,Carlos A Casiano (2012) Molecular cancer research : MCR.10
View All
Species Reactivity Key Applications Host Format Antibody Type
M, R WB Rabbit Purified Polyclonal Antibody
Description:
Anti-MeCP2 Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Upstate (Millipore)
Specificity:
Recognizes MeCP2, Mr 75 kDa. A protein at ~75 kDa was also observed in HeLa nuclear extract. This protein was competed by pre-incubation of the antibody with the immunogenic peptide, suggesting that some HeLa strains contain MeCP2 or a related protein, contrary to a previous report.
Molecular Weight:
~75 kDa observed
Immunogen:
KLH-conjugated, synthetic peptide corresponding to amino acids 465-478 of mouse MeCP2 (C-PRPNREEPVDSRTP) with an N-terminal cysteine added for conjugation purposes.
Isotype:
IgG
Background Information:
NA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, imprinting, and X chromosome inactivation. Methyl CpG binding proteins (MeCPs) specifically recognize methylated regions of DNA and repress transcription both directly and by association with known corepressor proteins which include members of the histone deacetylase protein families.
View All »
Species Reactivity:
Mouse
Rat
Species Reactivity Note:
Mouse and rat. Predicted to cross-react with human based on the conservation of the immunogenic sequence.
Control:
Mouse Brain lysate.
Quality Assurance:
Evaluated by Western Blot on Mouse Brain lysates.
Purification Method:
Protein A purfied
Presentation:
Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
P51608
Entrez Gene Number:
NP_004983
Gene Symbol:
AUTSX3
DKFZp686A24160
MRX16
MRX79
MRXS13
MRXSL
MeCP2
OTTHUMP00000026021
PPMX
RTS
RTT
View All »
Alternate Names:
MeCP-2 protein
mental retardation, X-linked 16
mental retardation, X-linked 79
methyl CpG binding protein 2
methyl CpG binding protein 2 (Rett syndrome)
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnosti |