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Nitric Oxide Fluorometric Assay Kit

产品编号: K252-200     查看说明书
产品名称: Nitric Oxide Fluorometric Assay Kit  .0   订购此产品 
供应商: Biovision
规格: 2 x 96 assays
目录价: 5175
库存状态: 3周到货
CAS编号:
应用范围: 生化实验
种属来源:
相关信息:

Nitric Oxide Fluorometric Assay Kit
Product Overview
Kit Summary:
• Detection method- Fluorescence (Ex/Em 380/461 nm)
• Sample type- Cell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Applications- Measurement of total nitrate/nitrite concentration
Features & Benefits:
• Simple procedure; takes only 2-3 hours
• Fast and convenient
• The fluorometric kit provides a simple two-step process for quantitative measurement of total nitrate/nitrite productions. The assay can be easily done in a 96-well plate.
Kit components:
• Assay Buffer
• Enzyme Cofactor
• Enhancer
• Nitrate Reductase
• Nitrate Standard
• Nitrite Standard
• DAN Probe
• Sodium Hydroxide
• Microtiter plate
• Plate Cover
Description:
Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. Since NO is rapidly converted to nitrite (NO²ˉ) and nitrate (NOᶟˉ), the total concentration of nitrite and nitrate is used as a quantitative measure of NO production. BioVision’s Nitric Oxide Fluorometric Assay Kit provides an accurate and convenient measurement of total nitrate/nitrite concentration in a simple two-step process. In the first step nitrate is converted to nitrite by nitrate reductase. In the second step, nitrite reacts with the fluorescent probe DAN (2, 3 diaminonaphthalene). NaOH enhances the fluorescent yield. The fluorescent intensity is proportional to the total nitric oxide production. The kit has been tested with culture media, plasma, and tissue homogenates.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.

保存条件: -20°C
说明书地址: 点击查看详细
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