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Glutathione Fluorometric Assay Kit
Product Overview
Kit Summary:
• Detection method- Fluorescence (Ex/Em 380/461 nm)
• Sample type- Cell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Applications- The Glutathione Detection Kit provides a simple in vitro assay for detecting the GSH changes in apoptosis and other pathological processes.
Features & Benefits:
• Simple two-step procedure; takes only 2 hours
• Fast and convenient
• The assay utilizes a dye that has a high affinity for glutathione. The unbound dye is almost nonfluorescent; whereas the dye fluoresces blue when bound to glutathione.
Kit components:
• Cell Lysis Buffer
• Monochlorobimane
• GST Reagent
• GSH Standard (1 mg; MW: 307)
Description:
Glutathione is the principal intracellular low-molecular-weight thiol that plays a critical role in the cellular defense against oxidative and nitrosative stress in mammalian cells. Diminished glutathione levels have been observed in the early stages of apoptosis. BioVision’s ApoGSH™ Glutathione Detection Kit provides a simple in vitro assay for detection of total glutathione changes in apoptosis and other samples. The assay utilizes monochlorobimane (MCB), a dye that appears to form an adduct exclusively with glutathione. The unbound MCB is almost nonfluorescent, whereas the dye fluoresces blue (Ex./Em. = 380nm/461nm) when bound to glutathione of reduced or oxidized form. The reaction is catalyzed by glutathione S-transferase. Thus, the amount of total glutathione can be easily detected using a fluorometer or a 96-well fluorometric plate reader.
Storage Conditions:
-70°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans. |