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DNA Damage Quantification Colorimetric Kit
Product Overview
Assay Kit Name:
DNA Damage Quantification Kit
Kit Summary:
• Detection method- Absorbance (450 and 650 nm)
• Species reactivity- Mammalian
• Applications- Kit provides all necessary reagents for convenient determination of DNA damage (abasic sites) in sample DNA
Sample Type:
Purified damaged DNA
Features & Benefits:
Kit provides all necessary reagents for 96-well plate format
Kit components:
• ARP Solution
• TE Buffer
• Glycogen Solution
• 0 ARP-DNA Standard
• 40 ARP-DNA Standard
• DNA Binding Solution
• HRP-Streptavidin
• 10X Wash Buffer
• HRP Developer
• 96-well Microplate (8 x 12 strips)
Description:
Apurinic/apyrimidinic (AP) sites are one of the major types of DNA lesions formed during
the course of base excision and repair of oxidized, deaminated or alkylated bases. It has
been estimated that about 2x105 base lesions are generated per cell per day. The level of
AP sites in cells can be a good indicator of DNA lesion and repair against chemical
damage and cell aging. The DNA Damage Quantification Kit utilizes the ARP (Aldehyde
Reactive Probe) reagent that reacts specifically with an aldehyde group which is the open
ring form of the AP sites. After treating DNA containing AP sites with ARP reagents, AP
sites are tagged with biotin residues, which can be quantified using avidin-biotin assay
followed by a colorimetric detection. The kit provides the necessary reagents for
convenient determination of abasic sites in purified DNA sample in 96-well plate format.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans. |