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Rat TNF-α ELISA Kit, EZRTNFA
Description:
Rat TNF-α ELISA Kit, EZRTNFA
Qty/Pk:
96-well strip plate
Background Information:
TNF-α (Tumor Necrosis Factor α), also termed Cachectin, Cytotoxic Factor (CF), Hemorrhagic Factor, Macrophage Cytotoxic Factor (MCF), is a potent multifunc-tional cytokine which can exert regulatory, inflammatory and cytotoxic effects on a wide range of normal lymphoid, non-lymphoid cells and tumor cells. TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells, and many transformed cell lines. It exists as a soluble 17.5 kD protein and a 26 kD mem-brane-associated protein. The soluble protein form derives from the membrane form by proteolytic processing. Dephosphorylation of the membrane occurs by binding to soluble TNFRSF1A/ TNFR1. TNF-α binding to surface receptors elicits a wide array of biological activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorrhagic necrosis of tumors in vivo, increased fibro-blast proliferation and enhanced chemotaxis and phagocytosis in neutrophils. It has been shown that TNF-α plays a critical role in many physiological and patho-logical conditions, including neurodegenerative diseases and cancer.
Rat TNF-α ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a capture antibody. This kit is specifically designed for the accurate quantification of Rat TNF-α from cell culture supernatant, serum, plasma and other biological fluids. It is analytically validated with ready-to-use reagents.
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Applications:
Rat TNF-α ELISA Kit, EZRTNFA -Cytokine ELISA.
Key Applications:
ELISA
Species Reactivity:
Rat
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Linearity of Dilution:
Contact Technical Service for this information
Sensitivity:
4.2 pg/mL
Product Name:
Rat TNF-α ELISA Kit, EZRTNFA
Specificity:
This kit recognizes mouse TNF-α and there is 0.04% cross reactivity with human TNF-a. No other cross reactivity was observed when this kit analyzed the following cytokines/chemokines, each at a concentration of 50 ng/ mL
Rat: IL-1a, IL-1b, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 (p70), IL- 13, IL-17A, IL-17E, IL-17F, IL-18, IL-22, IL-23, IL-33, CCL20, GM-CSF, IFN-g, KC, MCP-1, RANTES
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Analytes Available:
TNF-α
Precision, %:
Intra-assay: Less than 10 %
Inter-assay: Less than 15 %
Accuracy:
111% |