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Mouse IL-17F ELISA Kit, EZMIL17F
Description:
Mouse IL-17F ELISA Kit, EZMIL17F
Qty/Pk:
96-well strip plate
Background Information:
IL-17F is a member of the IL-17 cytokine family which consists of at least seven structurally related proteins (IL-17A, B, C, D, E, F, and A/F). IL-17F homodimer adopts a cystine knot motif formed through the interactions of four cysteines, one of which is responsible for inter-chain bonding. IL-17F is most closely related to IL-17A, sharing 50% amino acid sequence homology. IL-17F is expressed in activated Th17 cells, along with IL-17A homodimers and IL-17A/F heterodimers. It is also expressed in activated monocytes. IL-17F has been shown to inhibit endothelial cell angiogenesis and induce increased production of IL-2, TGF-b, and MCP-1, playing a critical role in the regulation of inflammatory reactions.
The Mouse IL-17F ELISA kit is a Sandwich Enzyme- Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a capture antibody.
This kit is specifically designed for the accurate quantification of mouse IL-17F from cell culture supernatant, serum, plasma and other biological fluids. This kit is analytically validated with ready-to-use reagents.
Key Applications:
ELISA
Species Reactivity:
Mouse
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Linearity of Dilution:
Contact Technical Service for this information
Sensitivity:
1.1 pg/mL
Product Name:
Mouse IL-17F ELISA Kit, EZMIL17F
Specificity:
The antibody pair used in this kit showed 2% cross reactivity when tested against mouse IL-17A/F at a concentration of 5 ng/mL. Rat IL-17F showed 1% cross-reactivity at a concentration of 10 ng/ mL.
No cross-reactivity was observed when this kit was used to analyze the following recombinant cytokines/chemokines at up to 50 ng/mL:
Human: IL-1a, IL-1b, IL-2, IL-6, IL-8, IL-13, IL-15, IL-17A, IL-17E, IL-17F, IL-22, IL-32a, IL-33, FGF-basic, IFN-g, TNF-a, TSLP
Mouse: IL-1a, IL-1b, IL-2, IL-3, IL-10, IL-13, IL-17A, IL-17B, IL-17E, IL-22, IL-33, FGF-basic, GM-CSF, IFN-g, MCP-1, SCF, TNF-a, TSLP
Rat: IL-2, IL-33, TNF-α
Analytes Available:
IL-17F
Precision, %:
Intra-assay: <10 %
Inter-assay: <15 %
Accuracy:
101% |