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THP1-NLRC4 cells
NLRC4 inflammasome test cell line
THP1-NLRC4 cells are derived from the THP1 human monocytic cell line, which represents the most commonly used model cell line to study inflammasome activation.
THP1-NLRC4 cells stably overexpress NLRC4 and naturally express TLR5. Stimulation of these cells with flagellin triggers TLR5 signaling leading to NF-κB activation and the production of pro-IL1β. Once in the cytosol, flagellin induces the formation of the NLRC4 inflammasome resulting in the activation of caspase-1 and the release of IL-1β.
Levels of IL-1β secreted into the supernatant of THP-1 cells can be monitored using the HEK-Blue™ KD-TLR5 cell line.
Antibiotic resistance: Blasticidin
Growth medium: RPMI 1640 (2 mM L-glutamine, 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate) with 10% heat-inactivated fetal bovine serum, 100 mg/ml Normocin™, Pen-Strep (50 U/ml-50 μg/ml)
Guaranteed mycoplasma-free
Shipped on dry ice
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