pFUSEN-hG1e2Fc pFUSEN-hG1e2Fc is a cloning plasmid for the generation of a Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the mutated human IgG1 heavy chain and the hinge region fused to the N-terminus of a protein of interest.. The engineered hIgG1e2 Fc contains the following mutations: M252Y/S254T/T256E and H433K/N434F. Human IgG1e2-Fc contains mutations in the site of interaction of IgG1 with FcRn that enhance the plasma half-life by increasing the affinity of IgG1 for FcRn. Isotype: IgG1 human Mutations: M252Y/S254T/T256E + H433K/N434F Characteristics: - Increased binding to neonatal FcR (FcRn) - Increased half-life Selectable with Zeocin™ in E. coli and mammalian cells. For transient or stable transfection.