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SIRT4 (GST-tagged), Human recombinant
Intracellular regulatory proteins with mono-ADP-ribosyltransferaseﾠactivity.
Product Overview
Product Name: SIRT4 (GST-tagged), Human recombinant
Alternate Name/Synonyms: SIR2-like Protein 4; NAD-dependent ADP-ribosyltransferase Sirtuin 4; SIR2L4; Silent Information Regulator 4; Sirtuin 4
Gene Symbol: SIRT4
Accession #: Q9Y6E7
Gene ID: 8350
Source: E. coli
Appearance: Liquid
Physical Form Description: 50 mM sodium phosphate, pH 7.2, 100 mM sodium chloride, 2.5 mM DTT, 20% glycerol.
Molecular Weight: 61.9 kDa (NT GST Tag)
Purity by SDS-PAGE: ≥95%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: N/A
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: Dry ice
Background Information: There are seven human sirtuins, also known as class III HDACs, which have been designated SIRT1 to SIRT7. Each is involved in various post-translational modifications by utilizing NAD dependent deacetylase and APD-ribosyltransferase activities. SIRT4 is a mitochondrial ADP-ribosyltransferase responsible for the transfer of ADP-ribose from NAD to specific substrates such as glutamate dehydrogenase (GDH). In caloric-sufficient conditions, SIRT4 ADP-ribosylates GDH, repressing its activity and correspondingly decreasing insulin secretion. There is some uncertainty about the relevance of sirtuin ribosyl transferase activity due to its very slow rate, which is about 500 times lower than the corresponding deacetylation reaction (for those that have been characterized). SIRT4 is found in many tissues, but is specifically enriched in the kidney, heart, brain, and liver.
Amino Acid Sequence: N/A
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans. |