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Sushi Peptide S3, bacterial recombinant
>90% Pure Recombinant Bacterial Sushi 3 peptide.
Product Overview
Product Name: Sushi Peptide S3, bacterial recombinant
Alternate Name/Synonyms: Factor C Sushi 3, Sushi 3
Gene Symbol: N/A
Accession #: N/A
Gene ID: N/A
Source: E. coli
Appearance: Lyophilized powder
Physical Form Description: Lyophilized from 50 mM Tris. HCl and 100 mM NaCl (pH 8.0)
Molecular Weight: 16.1 kDa
Purity by SDS-PAGE: ≥90%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: N/A
Reconstitution Instructions: Reconstitute with water. Recommended conc: 0.1 - 1 mg/ml.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: Biovision’s Sushi Peptide S3 is a trimer of one of the high endotoxin-binding domains, Sushi 3 (or S3) within Factor C, a lipopolysaccharide (LPS)-sensitive serine protease of the horseshoe crab (Limulus Polyphemus). S3 display detergent-like properties in disrupting LPS aggregates, with specificity for palmitoyl-oleoyl-phosphatidylglycerol (POPG) resulting from electrostatic and hydrophobic forces between the peptides and the bacterial lipids. During interaction with POPG, the S3 resumes a mixture of alpha-helix and beta-sheet structures. The unsaturated nature of POPG confers fluidity and enhances insertion of the peptides into the lipid bilayer, causing maximal disruption of the bacterial membrane. In short, peptide S3 can bind to lipopolysaccharide (LPS) and inhibit the growth of Gram-negative bacteria without affecting mammalian cells. It has been shown that endotoxin activates Factor C-based catalytic coagulation cascade resulting in the gelation of Limulus blood. This process is the basis of Limulus Amebocyte Lysate (LAL) endotoxin detection method.
Amino Acid Sequence: N/A
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans. |