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Human CellExp™ IFN-gamma, Human Recombinant
A central regulator of the immune response and signals
Product Overview
Product Name: IFN-gamma, Human CellExp™, human recombinant
Alternate Name/Synonyms: IFNG, IFG, IFI
Gene Symbol: IFN-gamma
Accession #: P01579
Gene ID: 3458
Source: HEK 293 cells
Appearance: Lyophilized powder
Physical Form Description: Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 100 mM NaCl, pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Molecular Weight: Calculated MW of 16.8 kDa with no tag. The predicted N-terminus is Gln 24. DTT-reduced protein migrates as 25.0 kDa due to glycosylation.
Purity by SDS-PAGE: ≥93%
Purity by HPLC: N/A
Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg) by LAL method
Biological Activity: Determined by a cytotoxicity assay using HT-29 cells. ED50 <0.05ng/ml, corresponding to a specific activity of >2X10^7 Unit/mg.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 100 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: Interferon-gamma (IFN-γ/IFNG) is a dimerized soluble cytokine that is the only member of the type II class of interferon. This interferon was originally called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFN-γ belongs. IFN-gamma has been used in a wide variety of clinical indications. Interferon-gamma (IFN gamma) is a central regulator of the immune response and signals via the Janus Activated Kinase (JAK)-Signal Transducer and Activator of Transcription (STAT) pathway. Interferon gamma has broader roles in activation of innate and adaptive immune responses to viruses and tumors, in part through upregulating transcription of genes involved in cell cycle regulation, apoptosis, and antigen processing/presentation. Despite this, rodent and human trophoblast cells show dampened responses to IFNG that reflect the resistance of these cells to IFNG-mediated activation of major histocompatibility complex (MHC) class II transplantation antigen expression.
Amino Acid Sequence: N/A
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans. |