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Human CellExp™ IL-10, Murine Recombinant
An anti-inflammatory cytokine
Product Overview
Product Name: IL-10, Human CellExp™, murine recombinant
Alternate Name/Synonyms: CSIF, IL-10, IL10A, TGIF, B-TCGF, GVHDS, MGC126450, MGC126451, RP11-262N9.1, Interleukin-10
Gene Symbol: IL-10
Accession #: P18893
Gene ID: 16153
Source: HEK 293 cells
Appearance: Lyophilized powder
Physical Form Description: Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 100 mM NaCl, pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Molecular Weight: Calculated MW of 19.2 kDa with no tag. The predicted N-terminus is Ser 19. DTT-reduced protein migrates as 22.0 kDa due to glycosylation.
Purity by SDS-PAGE: ≥95%
Purity by HPLC: N/A
Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg) by LAL method
Biological Activity: The bio-activity was determined by dose-dependent co-stimulation (with IL-4) of the proliferation of mouse MC/9 cells. The ED50 was < 2 ng/ml, corresponding to a specific activity of > 5 x 10^5 units/mg.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 100 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: Interleukin-10 (IL-10) is an also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. Mature human IL-10 shares 72% - 86% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells. IL-10 is capable of inhibiting synthesis of pro-inflammatory cytokines such as IFN-γ, IL-2, IL-3, TNFα and GM-CSF made by cells such as macrophages and regulatory T-cells. It also displays a potent ability to suppress the antigen-presentation capacity of antigen presenting cells. However, it is also stimulatory towards certain T cells and mast cells and stimulates B cell maturation and antibody production. Knockout studies suggested the function of Interleukin-10 / IL-10 as an essential immunoregulator in the intestinal tract. Patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant interleukin-10, showing the importance of interleukin-10 for counteracting excessive immunity in the human body.
Amino Acid Sequence: N/A
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans. |