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Human CellExp™ PCSK9, Human Recombinant
Plays a major regulatory role in cholesterol homeostasis
Product Overview
Product Name: PCSK9, Human CellExp™, human recombinant
Alternate Name/Synonyms: PCSK9, FH3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, Proprotein convertase subtilisin/kexin type 9
Gene Symbol: PCSK9
Accession #: Q8NBP7
Gene ID: 255738
Source: HEK 293 cells
Appearance: Lyophilized powder
Physical Form Description: Sterile filtered through a 0.22 micron filter. Lyophilized from 1 x PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Molecular Weight: This protein fused with polyhistidine tag at the C-terminus, has a calculated MW of 75.1 kDa. The predicted N-terminus is Gln 31. DTT-reduced Protein migrates as 20 kDa and 62 kDa due to glycosylation and proteolytic digestion.
Purity by SDS-PAGE: ≥97%
Purity by HPLC: N/A
Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg) by LAL method
Biological Activity: Measured by its ability to bind with human LDLR in a functional ELISA Immobilized recombinant human PCSK9 at 10 μg/ml (100 μl/well) can bind biotinylated human LDLR. The ED50 for this effect is typically 15 ng/ml.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 100 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Storage Temp.: -20°C
Shipping: Gel pack
Background Information: Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).
Amino Acid Sequence: N/A
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans. |