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SIRT1 (193-747 aa) (GST-tagged), Human recombinant
Regulates the activity of the p53 tumor suppressor and inhibits apoptosis
Product Overview
Product Name: SIRT1 (193-747 aa) (GST-tagged), Human recombinant
Alternate Name/Synonyms: NAD-dependent Deacetylase 1, SIR2L1, SIR2-like Protein 1, Sirtuin 1, Silent Information Regulator 2
Gene Symbol: SIRT1
Accession #: Q96EB6
Gene ID: 23411
Source: E. coli
Appearance: Liquid
Physical Form Description: Supplied as a solution in 50 mM sodium phosphate, pH 7.2, containing 100 mM sodium chloride and 20% glycerol
Molecular Weight: 82.9 kDa
Purity by SDS-PAGE: ≥60%
Purity by HPLC: N/A
Endotoxin Level: N/A
Biological Activity: ≥ 550 pmol/min/mg
Reconstitution Instructions: N/A
Storage Temp.: -80°C
Shipping: Dry Ice
Background Information: The sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs) and have been shown to catalyze a reaction that couples lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose from NAD+ and the abstracted acetyl group. There are seven human sirtuins, which have been designated SIRT1-7. SIRT1, which is located in the nucleus, is the human sirtuin with the greatest homology to yeast Sir2 (Silent information regulator 2) and has been shown to regulate the activity of the p53 tumor suppressor and inhibit apoptosis. These results have significant implications regarding an important role of SIRT1 in modulating the sensitivity of cells in the p53-dependent apoptotic response and the possible effect in cancer therapy. Since the growth suppressive function of p53 is strongly enhanced by DNA damaging reagents, it is expected that inhibitors of SIRT1 may be effective anti-cancer drugs.
Amino Acid Sequence: N/A
Handling: Centrifuge the vial prior to opening
USAGE: For Research Use Only! Not For Use in Humans. |