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Luciferase Reporter—Ready-To-Glow Secreted Luciferase Assay
The Ready-To-Glow Secreted Luciferase Assay is based on the secreted Metridia luciferase reporter. It combines the advantages of a live-cell assay with the sensitivity of an enzyme-based system.
Our Ready-To-Glow MetLuc Monoclonal Antibody makes it easy to detect Metridia luciferase expressed by mammalian cells, as well as recombinant Metridia luciferase, in amounts down to 20 ng using Western blot analysis.
Benefits of using a live cell assay
Ready-To-Glow Secreted Luciferase Reporter Systems Firefly and Renilla-Based Reporter Systems
One-step protocol, without cell lysis Yes. Reporter molecule is naturally secreted into the media and can be assayed without lysing cells. No.
2–4 fold higher signal than Renilla or firefly assays Yes. No.
Multiple data points per well Yes. Single well can be used for multiple time points, as there is no cell lysis. No.
Multiple compounds/trigger testing on same cells (single transfection) Yes. No.
Removal of background signal Yes. Background signal can be reset to zero by removing and replacing the media No.
Available Formats
•The Ready-To-Glow Secreted Luciferase Reporter System includes a promoterless plasmid and is designed to insert your promoter of interest upstream of the sequence-optimized Metridia secreted luciferase reporter gene.
•The Lenti-X Ready-To-Glow Secreted Luciferase Reporter System includes a promoterless vector with lentiviral delivery, and is designed to insert your promoter of interest upstream of the sequence-optimized Metridia secreted luciferase reporter gene.
•The NFkB Secreted Luciferase Reporter System is designed to monitor NFkappaB signal transduction pathways. Adding TNF-alpha, IL-1, or lymphokine receptor stimulants to the cell culture medium induces the binding of transcription factors to the enhancer element, thereby initiating transcription of the secreted Metridia luciferase reporter gene.
•The CRE Secreted Luciferase Reporter System is designed to monitor the activation of cAMP binding protein (CREB) and cAMP-mediated signal transduction pathways. For example, treatment of cells with cAMP or forskolin activates CREB or ATF to bind CRE, thus initiating transcription of the secreted Metridia luciferase reporter gene.
Dual Secreted Reporter Assay—Luciferase & SEAP
The Ready-To-Glow Dual Secreted Reporter System has two live cell reporters that do not require cell lysis. In addition to the secreted luciferase assay, it includes a secreted version of alkaline phosphatase. |