NF-kB Activation Inhibitor VIII, A-UBI

NEMO NOA Peptide, Cell-Permeable, A-UBI
NEMO Ubiquitin Binding Domain Inhibitor
A cell-permeable 37-mer peptide derived from the NEMO NOA ubiquitin binding site (294-314 of mouse sequence with D304R), and fused with an Antennapedia cell penetrating protein (A) at the N-terminus. It is shown to attenuate NF-κB activation via blocking interactions between K63-polyubiquitin chains and the NOA ubiquitin binding domain of NEMO (Kd = 17 µM for CC2-LZ/A-UBI complex). The biotin-labled A-UBI specifically inhibits the LPS-induced NF-κB activation (IC50 = 1.1 µM, compared with 23 µM for IKKβ derived A-NBD) and TNF-α induced NF-κB activation (IC50 = 21 µM) in 70Z/3-C3 pre-B lymphocytes. It displays an inhibitory effect on nuclear translocation of NF-κB p65 and IKK kinase activity at 10 µM, but does not significantly affect other signaling pathways such as MAPK/ERK, MAPK/JNK, NF-κB and NFAT.
产品信息
Peptide Sequence  RQIKIWFQNRRMKWKKLKAQADIYKARFQAERHAREK
Form  White powder
Molar mass  4758.59
Protect from Light  是的
Packaged under inert gas  是的
Purity  ≥95% by HPLC
Solubility  H2O
Chemical formula  C215H347N71O50S1 plus TFA and water
商店和运输信息
Storage  -20°C
Ship  Shipped with Blue Ice or with Dry Ice
Standard Handling