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产品名称:GenJet (Ver. II) DNA In Vitro Transfection Reagent for WEHI-231 Cell
功能:
GenJet™ DNA In Vitro Tranfection Reagent for WEHI-231 is pre-optimized for transfecting WEHI-231 cells. WEHI-231 is a murine B cell lymphoma that has been used extensively as a model for the immature stage B cell and its functional response to Ag receptor cross-linking as a model for immature B cell tolerance. This cell line expresses sIgM, CD5, and FcR gamma, but lacks the B cell- specific isoform of CD45 (B220).
最佳转染条件:
Confluency of cell on the day of shaving 95%
GenJet™ (µl) : DNA (µg) Ratio 3:1
Diluent for DNA and Transfection Reagent Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet™/DNA Complex 15 minutes at RT
Maximal Efficiency 48 hours
Transfection Results:
Reporter Gene EGFP
Plasmid pEGFP-N3 (CMV promoter)
Efficiency (GFP %) 66%
储存条件:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer. |