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产品名称:GenJet (Ver. II) DNA In Vitro Transfection Reagent for U-2 OS Cell
功能:
GenJet™ DNA In Vitro Tranfection Reagent for U2OS cells is pre-optimized for transfecting U2OS cells. The U2OS cell line, originally known as the 2T line, was cultivated from the bone tissue of a fifteen-year-old human female suffering from osteosarcoma. Established in 1964, the original cells were taken from a moderately differentiated sarcoma of the tibia. U2 OS cells exhibit epithelial adherent morphology and viruses were not detected in the line during co-cultivation with WI-38 cells or in CF tests against SV40, RSV, or adenoviruses. Cells are positive for insulin-like growth factor I (IGF-I) and insulin-like growth factor II (IGF II) receptors and express a number of antigens, including blood type A, Rh+, HLA A2, Aw30, B12, Bw35, and B40(+/-).
最佳转染条件:
Confluency of cell on the day of shaving 60~70%
GenJet™ (µl) : DNA (µg) Ratio 3:1
Diluent for DNA and Transfection Reagent Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet™/DNA Complex 15 minutes at RT
Maximal Efficiency 48 hours
Transfection Results:
Reporter Gene EGFP
Plasmid pEGFP-N3 (CMV promoter)
Efficiency (GFP %) 95%
储存条件:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer. |