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产品名称:GenJet (Ver. II) DNA In Vitro Transfection Reagent for MEFs
功能:
GenJet™ DNA In Vitro Tranfection Reagent for MEFs is pre-optimized and conditioned for transfecting Mouse Embryonic Fibroblasts (MEFs). MEFs are required to support the growth of undifferentiated mouse or human ES cells, iPS cells, thus they are also called feeder cells. MEFs are isolated from mouse embryos and are used at their early passages. Before MEFs are used as feeder cells, they must be treated by irradiation or mitomycin C to stop the cells from further dividing. However MEFs are nortoriously hard to transfect. With the pre-optimized reagent, you will get up to 85% GFP+ MEFs.
最佳转染条件:
Confluency of cell on the day of shaving 95~100%
GenJet™ (µl) : DNA (µg) Ratio 4:1
Diluent for DNA and Transfection Reagent Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet™/DNA Complex 12 minutes at RT
Maximal Efficiency 48 hours
Transfection Results:
Reporter Gene EGFP
Plasmid pEGFP-N3 (CMV promoter)
Efficiency (GFP %) 85%
储存条件:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer. |