GenJet In Vitro DNA Tranfection Reagent for PC-12 Cell

产品名称:GenJet In Vitro DNA Tranfection Reagent for PC-12 Cell
功能:
GenJet™ DNA In Vitro Tranfection Reagent for PC12 cell is pre-optimized for most efficiently transfecting PC12 cells. PC12 is a cell line derived from a pheochromocytoma of the rat adrenal medulla.  PC12 cells stop dividing and terminally differentiate when treated with nerve growth factor.  This makes PC12 cells useful as a model system for neuronal differentiation.
最佳转染条件:
Confluency of cell on the day of shaving 80%
GenJet™ (µl) : DNA (µg) Ratio   3:1
Diluent for DNA and Transfection Reagent Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet™/DNA Complex 15 minutes at RT
Maximal Efficiency     48 hours
Transfection Results:
Reporter Gene   EGFP
Plasmid    pEGFP-N3 (CMV promoter)
Efficiency (GFP %)  50%
储存条件:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.