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原装正品|GenMute siRNA Transfection Reagent for C2C12 Cell

产品编号: SL100568-C2C12     查看说明书
产品名称: 原装正品|GenMute siRNA Transfection Reagent for C2C12 Cell  .0   订购此产品 
供应商: SignaGen
规格: 1.0 ml
目录价: 1980
库存状态: 询盘
CAS编号:
应用范围: 转染试剂
种属来源:
相关信息:

原装正品|GenMute siRNA Transfection Reagent for C2C12 Cell
功能:
GenMute™ siRNA Transfection Reagent for C2C12 Cell is pre-optimized for transfecting siRNA to C2C12 cells. C2C12 cells were originally obtained by Yaffe and Saxel (1977) through selective serial passage of myoblasts cultured from the thigh muscle of C3H mice 70 h after a crush injury. These cells were shown to be capable of differentiation. C2C12 cells are a useful model to study the differentiation of non-muscle cells to skeletal muscle cells (e.g myosin phosphorylation mechanisms) and express muscle proteins and the androhen receptor (AR).
最佳转染条件:
Cell culture medium     DMEM with 4.5 g/L glucose, 10% FBS
Confluency of cell on the day of transfection  ~50%
Optimal siRNA concentration    40 nM
Diluent for siRNA and GenMute? Reagent   1xGenMute? Transfection Buffer
Incubation Time to Form GenMute?/siRNA Complex  ~15 minutes at RT
Maximal Silencing Efficiency     48 hours
Transfection Results:
Reporter Gene   Renilla Luciferase 
Silencing Efficiency (% ) 80%
储存条件:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.

保存条件: 4°C
说明书地址: 点击查看详细
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