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原装正品|GenMute siRNA Transfection Reagent for Hela Cell
功能:
GenMute™ siRNA Transfection Reagent for Hela Cell is pre-optimized for transfecting siRNA to Hela cells. GenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions based on transfecting 10 pmoles siRNA or miRNA mimics in 24-well plate.
Refer to the following optimal transfection conditions for maximal silencing on Hela cells. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or ~416 transfections in 6 well plates.
最佳转染条件:
Cell culture medium DMEM with 4.5 g/L glucose, 10% FBS
Confluency of cell on the day of transfection ~50%
Optimal siRNA concentration 20 nM
Diluent for siRNA and GenMute? Reagent 1xGenMute? Transfection Buffer
Incubation Time to Form GenMute?/siRNA Complex ~15 minutes at RT
Maximal Silencing Efficiency 48 hours
Transfection Results:
Reporter Gene Renilla Luciferase
Silencing Efficiency (% ) 90%
储存条件:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer. |